Culture filtrate proteome analysis of aminoglycoside resistant clinical isolates of Mycobacterium tuberculosis

Background Aminoglycosides are commonly used in tuberculosis treatment and are drugs of choice especially for MDR patients. They inhibit protein synthesis in susceptible bacteria by interacting with steps of translation. Several explanations have been put forward to explain the mechanism of aminoglycosides resistance but still our knowledge is fragmentary. Many culture filtrate proteins of pathogen represent potential targets for drugs, diagnostic probes and vaccine components; analysis of mycobacterial culture filtrate proteome in relation to aminoglycoside drug resistance is urgently required.